APOLIPOPROTEIN E (RS429358, RS7412) GENOTYPES AND PERIODONTITIS RISK IN SOUTH INDIAN POPULATION - AN ASSOCIATION ANALYSIS

Abstract

BACKGROUND: Periodontitis is a chronic inflammatory disease. Periodontal pathogens are screened by Toll-like receptors (TLRs), and
the inflammatory signaling pathway is regulated by apolipoprotein E (apoE).
AIM: The present study was to analyze the association of apoE (rs429358, rs7412) with stage II periodontitis, stage III/IV periodontitis, and healthy controls in the South Indian population.
MATERIALS AND METHODS: A total of 328 subjects were recruited for this study, among which 81 stage II periodontitis patients, 80
stage III/IV periodontitis patients, and 167 healthy controls. The genomic DNA was isolated from the peripheral blood. Three major
haplotypes of the apoE gene were genotyped in control samples and periodontitis cases using Real-Time polymerase chain reaction (RTPCR). The reaction was performed using the KAPASYBR® FASTqPCR Kit.
RESULTS: Statistical analysis was done using the R package. Data were analysed by independent sample t-test and χ2 test. Our study
showed a significant association between the E2/E3 genotype and stage II periodontitis in the South Indian population (OR 3.85; CI 1.03- 15.62; p = 0.046). For stage III/IV periodontitis, there was no significant association with the E2/E4 genotype in the South Indian population (OR 0.33; CI 0.07-1.15; p = 0.085).
CONCLUSION: The present study has demonstrated that the E4 allele and the E4/E4 genotype are more prevalent in the study population. The E2/E3 genotype was associated with stage II periodontitis in the South Indian population.